Microfluidic Paper-based Analytical Device for Fluorescence Detection of Lactoferrin in Tear Fluid
نویسندگان
چکیده
A microfluidic paper-based analytical device (μPAD) for the determination of lactoferrin in human tear fluid has been developed. By using the fluorescence signal emitted from lactoferrin-terbium complexes, an antibody-free sensing system has been achieved. Application of 2.5 μL of sample onto a μPAD allowed for the detection of lactoferrin concentration in human tear fluid. The selectivity for lactoferrin among the primary constituents of tear fluid has been confirmed. Furthermore, the accuracy of the developed μPAD has been evaluated by comparing the analysis results of real samples with the conventional ELISA method.
منابع مشابه
Paper-based microfluidic system for tear electrolyte analysis
The analysis of tear constituents at point-of-care settings has a potential for early diagnosis of ocular disorders such as dry eye disease, low-cost screening, and surveillance of at-risk subjects. However, current minimally-invasive rapid tear analysis systems for point-of-care settings have been limited to assessment of osmolarity or inflammatory markers and cannot differentiate between dry ...
متن کاملPaper-based microfluidic system for tear electrolyte analysis† †We declare no competing financial interests. ‡ ‡Electronic supplementary information (ESI) available: Microscopic images of G1 paper and G41 paper under brightfield; optimization of CO2 laser radiation fluence and beam speed for ablating filter paper-G1; photographs of DI water diffusion in microfluidic channels with different lengths, different widths, different viscosities of fluid and different numbers of channels; fluorescence intensity readouts of Na+ and K+ ions with varied concentrations of fluorescent probes; effect of variations in temperature on fluorescence intensity; photographs of DMSO on G1 paper dried in the air; calibration curves of electrolyte sensing on G1 paper using microplate reader measurement; calculation of sensitivity of the fluorescent sensors based on International Union of Pure and Applied Chemistry (IUPAC) guidelines; quantification of ion interference in buffer solution and artificial tear fluid; light attenuation of LED lights using different optical filters; the design of the sample collection device and its potential clinical use; calibration curves of electrolyte sensors using the paper-based microfluidic system; quantifications of evaporation effect on sampling process; design of the sample collection device and its potential clinical use; batch-to-batch variation experiments; equation for background subtraction; movies of sample collection and measurements. See DOI: 10.1039/c6lc01450j Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file.
The analysis of tear constituents at point-of-care settings has a potential for early diagnosis of ocular disorders such as dry eye disease, low-cost screening, and surveillance of at-risk subjects. However, current minimally-invasive rapid tear analysis systems for point-of-care settings have been limited to assessment of osmolarity or inflammatory markers and cannot differentiate between dry ...
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